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DOI https://doi.org/10.1007/s10815-017-0891-9

Effect of GnRHa ovulation trigger dose on follicular fluid characteristics and granulosa cell gene expression profiles

Thi Ngoc Lan Vuong1,2, M. T. Ho2,3, T. Q. Ha2, M. Brehm Jensen4, C. Yding Andersen4, P. Humaidan5,6

  1. Department of Obstetrics and GynaecologyUniversity of Medicine and Pharmacy HCMCHo Chi Minh CityVietnam
  2. IVFMDMy Duc HospitalHo Chi Minh CityVietnam
  3. Research Center for Genetics and Reproductive Health (CGRH), School of MedicineVietnam National University HCMCHo Chi Minh CityVietnam
  4. Laboratory of Reproductive Biology, The Copenhagen University Hospital and Faculty of Health ScienceCopenhagen UniversityCopenhagenDenmark
  5. The Fertility ClinicSkive Regional HospitalSkiveDenmark
  6. Faculty of Health, Aarhus University and Faculty of HealthUniversity of Southern DenmarkAarhusDenmark
Abstract

Purpose
A recent dose-finding study showed no significant differences in number of mature oocytes, embryos and top-quality embryos when triptorelin doses of 0.2, 0.3 or 0.4 mg were used to trigger final oocyte maturation in oocyte donors co-treated with a gonadotropin-releasing hormone (GnRH) antagonist. This analysis investigated whether triptorelin dosing for triggering final oocyte maturation in oocyte donors induced differences in follicular fluid (FF) hormone levels and granulosa cell gene expression.
Methods
This single-centre, randomised, parallel, investigator-blinded trial was conducted in oocyte donors undergoing a single stimulation cycle at IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam, from August 2014 to March 2015. A total of 165 women aged 18–35 years with body mass index <28 kg/m2, anti-Müllerian hormone >1.25 ng/mL, and antral follicle count ≥6 were randomised to three different triptorelin doses for trigger. The main outcome was concentration of steroid hormones in FF collected from the first punctured follicle on each side. Moreover, luteinising hormone receptor (LHR), 3β-hydroxy-steroid-dehydrogenase (3ßHSD) and inhibin-Ba (INHB-A) gene expression in cumulus and mural granulosa cells were investigated in a subset of women from each group.
Results
Progesterone and oestradiol levels in FF did not differ significantly by trigger doses; findings were similar for 3βHSD, LHR and INHB-A gene expression in both cumulus and mural granulosa cells.
Conclusions
In women co-treated with a GnRH antagonist, no significant differences in FF steroid levels and granulosa cell gene expression were seen when different triptorelin doses were used to trigger final oocyte maturation.

KEYWORDS:

In vitro fertilisation Gonadotropin-releasing hormone agonist trigger LHR gene expression Triptorelin Follicular fluid

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