science-product-detail
DOI: https://doi.org/10.1007/s10815-019-01677-6
Live births after oocyte in vitro maturation with a prematuration step in women with polycystic ovary syndrome
Lan N. Vuong1,2,3 & Anh H. Le2,3 & Vu N. A. Ho2,3 & Toan D. Pham2,3 & Flor Sanchez4,5 & Sergio Romero4,5 & Michel De Vos4 & Tuong M. Ho2,3 & Robert B. Gilchrist6 & Johan Smitz4
Purpose
Standard oocyte in vitro maturation (IVM) usually results in lower pregnancy rates than in vitro fertilization (IVF). IVM preceded by a prematuration step improves the acquisition of oocyte developmental competence and can enhance embryo quality (EQ). This study evaluated the effectiveness of a biphasic culture system incorporating prematuration and IVM steps (CAPAIVM) versus standard IVM in women with polycystic ovarian morphology (PCOM).
Methods
Eighty women (age < 38 years, ≥ 25 follicles of 2–9 mm in both ovaries, no major uterine abnormalities) were randomized to undergo CAPA-IVM (n = 40) or standard IVM (n = 40). CAPA-IVM uses two steps: a 24-h prematuration step with C-type natriuretic peptide-supplemented medium, then 30 h of culture in IVM media supplemented with follicle-stimulating hormone and amphiregulin. Standard IVM was performed using routine protocols.
Result
A significantly higher proportion of oocytes reached metaphase II at 30 h after CAPA-IVM versus standard IVM (63.6 vs 49.0; p < 0.001) and the number of good quality embryos per cumulus-oocyte complex tended to be higher (18.9 vs 12.7; p = 0.11). Clinical pregnancy rate per embryo transfer was 63.2% in the CAPA IVM versus 38.5% in the standard IVM group (p = 0.04). Live birth rate per embryo transfer was not statistically different between the CAPA-IVM and standard IVM groups (50.0 vs 33.3% [p = 0.17]). No malformations were reported and birth weight was similar in the two treatment groups.
Conclusions
Use of the CAPA-IVM system significantly improved maturation and clinical pregnancy rates versus standard IVM in patients with PCOM. Furthermore, live births after CAPA-IVM are reported for the first time.
KEYWORDS:
In vitro fertilization . In vitro maturation . Polycystic ovary syndrome . Oocyte prematuration . C-type natriuretic peptide
Download
Similar articles
-
Review COVID-19 Pandemic Impacts Decision-Making and Psychosocial Behavior in Women Seeking Fertility Treatment in APAC—an ASPIRE Patient Experience Report
[ Budi Wiweko1,*, Tuong M. Ho2, Atsushi Tanaka3, Vanessa Raggio4, Jung-Ryeol Lee5, Milind Patil6, Carina Chi-Wai Chan7, Teraporn Wutayavanich8, Feiyang Diao9, Suresh Nair10,Rudie Frederick Mendiola11 ]
Background: ASPIRE (Asia-Pacific Initiative on Reproduction) aims to develop a “patient-ce...
-
Review Corifollitropin alfa followed by highly purified HMG versus recombinant FSH in young poor ovarian responders: A multicentre randomized controlled clinical trial
[ Panagiotis Drakopoulos1,2, Thi Ngoc Lan Vuong3,4, Ngoc Anh Vu Ho4, Alberto Vaiarelli1, Manh Tuong Ho4,5, Christophe Blockeel1,2,6, Michel Camus2, Anh Tuan Lam4, Arne van de Vijver1,2, Peter Humaidan8, Herman Tournaye1,2, and Nikolaos P. Polyzos1,2,7,8,* ]
STUDY QUESTION
Does administration of corifollitropin alfa followed by highly purified (hp) HMG result in higher... -
Review Micronized progesterone plus dydrogesterone versus micronized progesterone alone for luteal phase support in frozen-thawed cycles (MIDRONE): a prospective cohort study
[ Lan N. Vuong 1,2,*, Toan D. Pham2, Khanh T.Q. Le2, Trung T. Ly2, Ho L. Le2, Diem T.N. Nguyen2, Vu N.A. Ho2, Vinh Q. Dang2, Tuan H. Phung2, Robert J. Norman3, Ben W. Mol4,5, and Tuong M. Ho2 ]
STUDY QUESTION
Does the addition of oral dydrogesterone to vaginal progesterone as luteal phase support improve pregnancy...
-
Review Corifollitropin alfa vs recombinant FSH for controlled ovarian stimulation in women aged 35–42 years with a body weight ≥50 kg: a randomized controlled trial
[ N.L. Vuong1,2,*, D.T. Pham2, H.T. Phung2, H.N. Giang2, G.B. Huynh2, T.T.L. Nguyen2, and M.T. Ho2,3 ]
STUDY QUESTION
Is corifollitropin alfa 150 μg equivalent to follitropin beta 300 IU/day for controlled ovarian... -
Review Effect of GnRHa ovulation trigger dose on follicular fluid characteristics and granulosa cell gene expression profiles
[ Thi Ngoc Lan Vuong1,2, M. T. Ho2,3, T. Q. Ha2, M. Brehm Jensen4, C. Yding Andersen4, P. Humaidan5,6 ]
Purpose
A recent dose-finding study showed no significant differences in number of mature oocytes, embryos and to...