Predictors of twin pregnancy in in vitro fertilization with intracytoplasmic sperm injection cycles with day 3 double embryo transfer

Published: December 27, 2023

DOI: https://doi.org/10.5653/cerm.2023.06156

Duy Le Nguyen¹, Hieu Le-Trung Hoang^1,2, Vu Ngoc-Anh Ho^1,2, Toan Duong Pham¹, Nam Thanh Nguyen^1,2, Van Thi-Thu Tran^1,2, Tuong Manh Ho^1,2, Lan Ngoc Vuong^1,2,3

Objective

The purpose of this study was to identify factors associated with twin pregnancy following day 3 double embryo transfer (DET).

Methods

This retrospective cohort study incorporated data from 16,972 day 3 DET cycles. The participants were women aged between 18 and 45 years who underwent in vitro fertilization with intracytoplasmic sperm injection (IVF/ICSI) at My Duc Assisted Reproduction Technique Unit (IVFMD), My Duc Hospital, located in Ho Chi Minh City, Vietnam.

Results

Of the 16,972 day 3 DET cycles investigated, 8,812 (51.9%) resulted in pregnancy. Of these, 6,108 cycles led to clinical pregnancy, with 1,543 (25.3% of clinical pregnancies) being twin pregnancies. Factors associated with twin pregnancy included age under 35 years (odds ratio [OR], 1.5; 95% confidence interval [CI], 1.32 to 1.71; p<0.001) and cycles involving the transfer of at least one grade I embryo. Relative to the transfer of two grade III embryos, the risk of twin pregnancy was significantly elevated following the transfer of two grade I embryos (OR, 1.40; 95% CI, 1.16 to 1.69; p<0.001) or a combination of one grade I and one grade II embryo (OR, 1.27; 95% CI, 1.05 to 1.55; p=0.001).

Conclusion

By analyzing a large number of IVF/ICSI cycles, we identified several predictors of twin pregnancy. These findings can assist medical professionals in tailoring treatment strategies for couples with infertility.

Keywords: Cleavage stage embryos; Double embryo transfer; Embryo transfer; Fertilization in vitro; Pregnancy, twin