Support: (+84) 28 7308 5885 - (Ext) 303 or 310

Effect of single versus grouped culture of human cumulus–oocyte complexes in PCOS women treated with biphasic in vitro maturation: A sibling oocyte pilot study

Published: June 7th, 2024

Huy H. Pham1,2 | Anh H. Le1,2 | Tri C. Nguyen2 | Mai P. Q. Ma3 | Nazli Akin3 |Toan D. Pham2 | Minh H. N. Nguyen2 | Ho L. Le1,2 | Bao G. Huynh2,4 |Johan Smitz3 | Tuong M. Ho2,4 | Lan N. Vuong5

Authors information

1 IVMFD Phu Nhuan, My Duc Phu NhuanHospital, Ho Chi Minh City, Vietnam

2 HOPE Research Center, My Duc Hospital,Ho Chi Minh City, Vietnam

3 Follicle Biology Laboratory, FreeUniversity of Brussels (VUB), Brussels,Belgium

4 IVFMD, My Duc Hospital, Ho Chi MinhCity, Vietnam

5 Department of Obstetrics andGynecology, University of Medicine andPharmacy at Ho Chi Minh City, Ho ChiMinh City, Vietnam



This study investigated the differences in the maturation rate of single versus grouped cumulus–oocyte complexes (COCs) culture methods for capacitation in vitro maturation (CAPA-IVM) in women with polycystic ovary syndrome (PCOS).


This study was performed at My Duc Phu Nhuan Hospital, Vietnam from October 1, 2020 to October 24, 2021. Women aged 18–37 years with a diagnosis of PCOS were recruited. COCs from each woman were randomly divided into two groups: single or grouped culture during CAPA-IVM culture. The primary outcome was the maturation rate.


A total of 322 COCs from 15 eligible women included were randomly assigned to the two study groups. The maturation rate was comparable between the single and grouped culture groups (61.3% vs. 64.8%; p = 0.56). There were no significant differences in the number of 2-pronuclei fertilized oocytes, number of day-3 embryos, and number of good-quality embryos in the two culture method groups. In the single culture group, COCs morphology was associated with the day-3 embryo formation rate but not the maturation rate.


Comparable oocyte maturation and embryology outcomes between single and grouped COCs culture utilizing sibling COCs derived from women with PCOS suggest the feasibility of both methods for CAPA-IVM culture.