Support: (+84) 28 7308 5885 - (Ext) 303 or 310

Live births after oocyte in vitro maturation with a prematuration step in women with polycystic ovary syndrome

J Assist Reprod Genet 37: 347–357 (2020) – 2020-01-04

Lan N. Vuong1,2,3 & Anh H. Le2,3 & Vu N. A. Ho2,3 & Toan D. Pham2,3 & Flor Sanchez4,5 & Sergio Romero4,5 & Michel De Vos& Tuong M. Ho2,3 & Robert B. Gilchrist& Johan Smitz4

Published: 4 January, 2020

Author information


Standard oocyte in vitro maturation (IVM) usually results in lower pregnancy rates than in vitro fertilization (IVF). IVM preceded by a prematuration step improves the acquisition of oocyte developmental competence and can enhance embryo quality (EQ). This study evaluated the effectiveness of a biphasic culture system incorporating prematuration and IVM steps (CAPAIVM) versus standard IVM in women with polycystic ovarian morphology (PCOM).
Eighty women (age < 38 years, ≥ 25 follicles of 2–9 mm in both ovaries, no major uterine abnormalities) were randomized to undergo CAPA-IVM (n = 40) or standard IVM (n = 40). CAPA-IVM uses two steps: a 24-h prematuration step with C-type natriuretic peptide-supplemented medium, then 30 h of culture in IVM media supplemented with follicle-stimulating hormone and amphiregulin. Standard IVM was performed using routine protocols.
A significantly higher proportion of oocytes reached metaphase II at 30 h after CAPA-IVM versus standard IVM (63.6 vs 49.0; p < 0.001) and the number of good quality embryos per cumulus-oocyte complex tended to be higher (18.9 vs 12.7; p = 0.11). Clinical pregnancy rate per embryo transfer was 63.2% in the CAPA IVM versus 38.5% in the standard IVM group (p = 0.04). Live birth rate per embryo transfer was not statistically different between the CAPA-IVM and standard IVM groups (50.0 vs 33.3% [p = 0.17]). No malformations were reported and birth weight was similar in the two treatment groups.
Use of the CAPA-IVM system significantly improved maturation and clinical pregnancy rates versus standard IVM in patients with PCOM. Furthermore, live births after CAPA-IVM are reported for the first time.


In vitro fertilization . In vitro maturation . Polycystic ovary syndrome . Oocyte prematuration . C-type natriuretic peptide